60101
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- 中文名稱: 葡萄糖脫氫酶
- 英文名稱: Glucose Dehydrogenase
The enzyme is useful for the determination of D-glucose in clinical analysis and self-monitoring blood glucose meters.
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- 應 用: 生化診斷
- 產品類別: 酶
- 生化診斷: 比色法
規格
| Appearance | yellow lyophilizate |
| Activity | ≧700 U/mg |
| Contaminants | NAD glucose dehydrogenase ?1.0×10-2 U/U％ |
| Hexokinase ?1.0×10-2 U/U％ | |
| α-glucosidase ?1.0×10-2 U/U％ | |
| β-glucosidase ?1.0×10-2 U/U％ | |
| Filterability | ≧90% |
| Storage condition | below -20℃ protected from light |
特性
| Molecular weight | ca. 85 kDa (SDS-PAGE) |
| Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
| Michaelis constant | 2.2×10-2M (D-glucose) |
| pH Optimum | 7.0–7.5 |
| pH Stability | 4.5–8.0 |
| Optimum temperature | 40–50℃ |
| Thermal stability | below 45℃ |
| Specificity | D-glucose (100), maltose (?0.01), |
| D-xylose (8.7), D-galactose (0.7) , | |
sucrose (0.1) ,D-mannnose (3.9), 2-deoxy-d-glucose (52.2) |
應用
The enzyme is useful for the determination of D-glucose in clinical analysis and self-monitoring blood glucose meters.
參考文獻
Hatada M, Loew N, Inose-Takahashi Y, Okuda-Shimazaki J, Tsugawa W, Mulchandani A, Sode K (2018)
Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference.
Bioelectrochemistry, 121, 185-190
Okuda-Shimazaki, J, Yoshida H, Sode K (2020)
FAD dependent glucose dehydrogenases – Discovery and engineering of representative glucose sensing enzymes –
Bioelectrochemistry, 132, 107414
