60266
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- 中文名稱: 膽固醇氧化酶
- 英文名稱: Cholesterol Oxidase
The enzyme is useful for the determination of cholesterol in clinical analysis.
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- 應 用: 生化診斷
- 產品類別: 酶
- 生化診斷: 比色法
規格
| Appearance | yellow liquid | |
| Activity | ≧200 U/mL | |
| Contaminants | Catalase ≦2.0 U/U% | |
| pH | 7.3–7.7 | |
| Stabilizer | sucrose | |
| Storage condition | below -20℃ protected from light |
特性
| Molecular weight | ca. 59 kDa (gel filtration) |
| Structure | monomer of 60 kDa (SDS-PAGE) |
| Michaelis constant | 1.9×10-5 M (cholesterol) |
| pH Optimum | 6.5–8.0 |
| pH Stability | 3.5–8.5 |
| Optimum temperature | 55℃–65℃ |
| Thermal stability | below 70℃ |
| Stability (liquid form) | stable at 25℃ for at least one month |
| Inhibitors | Ag+, Hg2+ |
應用
The enzyme is useful for the determination of cholesterol in clinical analysis.
參考文獻
Aono, R. et al., Appl. Environ. Microbiol., 60, 2518–2523 (1994).
Doukyu, N. et al., Appl. Environ. Microbiol., 64, 1929–1932 (1998).
Doukyu, N. and Aono, R., Biochem. J., 341, 621–627 (1999).
Doukyu, N. and Aono, R., Appl. Microbiol. Biotechnol., 57, 146–152 (2001).
Aunpad, R. et al., Acta Cryst., D58, 2182–2183 (2002).
