61170
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- 中文名稱: 乳酸脫氫酶
- 英文名稱: Lactate Dehydrogenase
The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.
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- 應(yīng) 用: 生化診斷
- 產(chǎn)品類別: 酶
- 生化診斷: 比色法
規(guī)格
| Appearance | white lyophilizate |
| Activity | ≧150 U/mg |
| Storage condition | below -20℃ |
特性
| Molecular weight | ca. 115 kDa (gel filtration) |
| Michaelis constant | 2.5×10½M (lactate) 1.0×10¼M (pyruvate) |
| pH Optimum | 7.4–8.0 |
| pH Stability | 7.0–7.5 |
| Optimum temperature | 50–55℃ |
| Thermal stability | below 45℃ |
應(yīng)用
The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.
參考文獻(xiàn)
Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).
